FANCD2 polyclonal antibody
产品名称： FANCD2 polyclonal antibody
英文名称： FANCD2 polyclonal antibody
产品编号： PAB12428
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against partial recombinant FANCD2.
- Immunogen:
- Recombinant protein corresponding to N-terminus of human FANCD2.
- Host:
- Rabbit
- Reactivity:
- Human, Mouse
- Specificity:
- This antibody is specific to FANCD2.
- Form:
- Liquid
- Storage Buffer:
- In Tris-glycine, 150 mM NaCl (0.05% sodium azide)
- Storage Instruction:
- Store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing.
- Quality Control Testing:
- Antibody Reactive Against Recombinant Protein.
- Recommend Usage:
- Immunofluorescence (1:200-1:500)
Western Blot (1:10000-1:20000)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (2.5-5 ug/mL)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Defective mitochondrial peroxiredoxin-3 results in sensitivity to oxidative stress in Fanconi anemia.
Mukhopadhyay SS, Leung KS, Hicks MJ, Hastings PJ, Youssoufian H, Plon SE.J Cell Biol. 2006 Oct 23;175(2):225-35.
- 2.
- Young patients with oral squamous cell carcinoma: study of the involvement of GSTP1 and deregulation of the Fanconi anemia genes.
Tremblay S, Pintor Dos Reis P, Bradley G, Galloni NN, Perez-Ordonez B, Freeman J, Brown D, Gilbert R, Gullane P, Irish J, Kamel-Reid S.Arch Otolaryngol Head Neck Surg. 2006 Sep;132(9):958-66.
- 3.
- Spatial organization of the mammalian genome surveillance machinery in response to DNA strand breaks.
Bekker-Jensen S, Lukas C, Kitagawa R, Melander F, Kastan MB, Bartek J, Lukas J.J Cell Biol. 2006 Apr 24;173(2):195-206. Epub 2006 Apr 17.
- 4.
- Nbs1 is required for ATR-dependent phosphorylation events.
Stiff T, Reis C, Alderton GK, Woodbine L, O'Driscoll M, Jeggo PA.EMBO J. 2005 Jan 12;24(1):199-208. Epub 2004 Dec 16.
- 5.
- Repair kinetics of genomic interstrand DNA cross-links: evidence for DNA double-strand break-dependent activation of the Fanconi anemia/BRCA pathway.
Rothfuss A, Grompe M.Mol Cell Biol. 2004 Jan;24(1):123-34.
- Applications
- Western Blot (Cell lysate)
- Western blot analysis of FANCD2 in HeLa whole cell extract using FANCD2 polyclonal antibody (Cat # PAB12428).
- Immunohistochemistry
- Staining of human prostate, glandular epithelium using FANCD2 polyclonal antibody (Cat # PAB12428).
- Immunofluorescence
- FANCD2 colocalizes in vivo with another protein in SiHa cells after cell exposure to IR. Proliferating SiHa cells were exposed to 10 Gy of IR and double -color immunofluorescence staining was performed after 8 h. FANCD2 were stained with FANCD2 polyclonal antibody (Cat # PAB12428).
- Immunofluorescence
- FANCD2 polyclonal antibody (Cat # PAB12428) was used to stain FANCD2. FANCD2 colocalizes in vivo with another protein in U-2 OS cells after cell exposure to IR. Proliferating U-2 OS cells were exposed to 10 Gy of IR and double -color immunofluorescence staining was performed after 8 h.
- Entrez GeneID:
- 2177
- Protein Accession#:
- Q9BXW9
- Gene Name:
- FANCD2
- Gene Alias:
- DKFZp762A223,FA-D2,FA4,FACD,FAD,FAD2,FANCD,FLJ23826
- Gene Description:
- Fanconi anemia, complementation group D2
- Omim ID:
- 227646
- Gene Ontology:
- Hyperlink
- Gene Summary:
- The Fanconi anemia complementation group (FANC) currently includes FANCA, FANCB, FANCC, FANCD1 (also called BRCA2), FANCD2, FANCE, FANCF, FANCG, FANCI, FANCJ (also called BRIP1), FANCL, FANCM and FANCN (also called PALB2). The previously defined group FANCH is the same as FANCA. Fanconi anemia is a genetically heterogeneous recessive disorder characterized by cytogenetic instability, hypersensitivity to DNA crosslinking agents, increased chromosomal breakage, and defective DNA repair. The members of the Fanconi anemia complementation group do not share sequence similarity; they are related by their assembly into a common nuclear protein complex. This gene encodes the protein for complementation group D2. This protein is monoubiquinated in response to DNA damage, resulting in its localization to nuclear foci with other proteins (BRCA1 AND BRCA2) involved in homology-directed DNA repair. Alternative splicing results in two transcript variants encoding different isoforms. [provided by RefSeq
- Other Designations:
- Fanconi anemia complementation group D2,type 4 Fanconi pancytopenia
- Related Disease
- Adenocarcinoma
- Ataxia telangiectasia
- Breast cancer
- Breast Neoplasms
- Colorectal Neoplasms
- Genetic Predisposition to Disease
- Head and Neck Neoplasms
- Meningeal Neoplasms
- Meningioma
- Neoplasm Recurrence, Local
- Neoplasms, Glandular and Epithelial
- Neoplasms, Second Primary
- Ovarian cancer
- Ovarian Neoplasms
- Pancreatic Neoplasms
- Prostatic Neoplasms
- Tobacco Use Disorder
- Urinary Bladder Neoplasms
- Werner syndrome