SIGLEC12 polyclonal antibody
产品名称： SIGLEC12 polyclonal antibody
英文名称： SIGLEC12 polyclonal antibody
产品编号： PAB3649
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of SIGLEC12.
- Immunogen:
- A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human SIGLEC12.
- Host:
- Rabbit
- Reactivity:
- Human
- Form:
- Liquid
- Purification:
- Protein G purification
- Storage Buffer:
- In PBS (0.09% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment.
Clark HF, Gurney AL, Abaya E, Baker K, Baldwin D, Brush J, Chen J, Chow B, Chui C, Crowley C, Currell B, Deuel B, Dowd P, Eaton D, Foster J, Grimaldi C, Gu Q, Hass PE, Heldens S, Huang A, Kim HS, Klimowski L, Jin Y, Johnson S, Lee J, Lewis L, Liao D, Mark M, Robbie E, Sanchez C, Schoenfeld J, Seshagiri S, Simmons L, Singh J, Smith V, Stinson J, Vagts A, Vandlen R, Watanabe C, Wieand D, Woods K, Xie MH, Yansura D, Yi S, Yu G, Yuan J, Zhang M, Zhang Z, Goddard A, Wood WI, Godowski P, Gray A.Genome Res. 2003 Oct;13(10):2265-70. Epub 2003 Sep 15.
- 2.
- A second uniquely human mutation affecting sialic acid biology.
Angata T, Varki NM, Varki A.J Biol Chem. 2001 Oct 26;276(43):40282-7. Epub 2001 Aug 23.
- 3.
- Identification and characterization of S2V, a novel putative siglec that contains two V set Ig-like domains and recruits protein-tyrosine phosphatases SHPs.
Yu Z, Lai CM, Maoui M, Banville D, Shen SH.J Biol Chem. 2001 Jun 29;276(26):23816-24. Epub 2001 Apr 27.
- Applications
- Western Blot (Cell lysate)
- The SIGLEC12 polyclonal antibody (Cat # PAB3649) is used in Western blot to detect SIGLEC12 in Jurkat cell lysate.
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with SIGLEC12 polyclonal antibody (Cat # PAB3649) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma.
- Entrez GeneID:
- 89858
- Protein Accession#:
- Q96PQ1
- Gene Name:
- SIGLEC12
- Gene Alias:
- FLJ38600,S2V,SIGLECL1,SLG,Siglec-12,Siglec-L1,Siglec-XII
- Gene Description:
- sialic acid binding Ig-like lectin 12
- Omim ID:
- 606094
- Gene Ontology:
- Hyperlink
- Gene Summary:
- Sialic acid-binding immunoglobulin-like lectins (SIGLECs) are a family of cell surface proteins belonging to the immunoglobulin superfamily. They mediate protein-carbohydrate interactions by selectively binding to different sialic acid moieties present on glycolipids and glycoproteins. This gene encodes a member of the SIGLEC3-like subfamily of SIGLECs. Members of this subfamily are characterized by an extracellular V-set immunoglobulin-like domain followed by two C2-set immunoglobulin-like domains, and the cytoplasmic tyrosine-based motifs ITIM and SLAM-like. The encoded protein, upon tyrosine phosphorylation, has been shown to recruit the Src homology 2 domain-containing protein-tyrosine phosphatases SHP1 and SHP2. It has been suggested that the protein is involved in the negative regulation of macrophage signaling by functioning as an inhibitory receptor. This gene is located in a cluster with other SIGLEC3-like genes on 19q13.4. Alternatively spliced transcript variants encoding distinct isoforms have been described for this gene. [provided by RefSeq
- Other Designations:
- SIGLEC-like 1,sialic acid binding immunoglobulin-like lectin-like protein 1
- Related Disease